GACPAT HIV-1+2 - A SIMPLE, INEXPENSIVE ASSAY TO SCREEN FOR, AND DISCRIMINATE BETWEEN, ANTI-HIV-1 AND ANTI-HIV-2

A simple and cheap assay suitable for screening for anti-HIV 1 and anti-HIV 2 and discriminating between them was evaluated. In it specimens are incubated in U-bottomed microplate wells coated with anti-human IgG for 30 min at room temperature. After washing, 100 mu l of a 1 in 50 dilution of HIV 1-coated gelatin particles (Serodia-HIV 1/2, Fujirebio) are added. Settling patterns are read on the second day: A positive reaction is indicated by adherence of the particles and a negative by a button. The HIV 1 particles a re then washed away a nd HIV 2 particles added. Anti-HIV 2 reaction patterns are read on the third day. To assess the performance of the modified ''GACPAT HIV 1+2'' assay a panel of 1,621 serum/plasma specimens was used. It comprised validated anti-HIV 1 positive (n = 220), anti-HIV 2 positive (n = 214), dual anti-HIV 1/antiHIV 2 positive (n = 11), and anti-HIV negative (n = 1,176) serum/plasma specimens. All 434 specimens that contained anti-HIV 1 or anti-HIV 2 reacted positively with the homologous particles. The 11 dually positive specimens reacted positively with both HIV 1 and HIV 2 particles. Five (2.3%) anti-HIV 1 and five (2.3%) anti-HIV 2 positive specimens gave positive reactions with both particle types, but none of the five cross-reactive anti-HIV 2 specimens were dually reactive when the order of particle addition was reversed. One repeat false positive reaction was recorded with the HIV 1 particles and none with the HIV 2 particles, giving specificities of 99.9% and 100%, respectively. Type specificity was 98.8% after the results of the reverse assay had been taken into account. The assay was sensitive for anti-HIV 1 in seroconversion series and for anti-HIV 2 in highly diluted specimens. GACPAT HIV 1 + 2 is an accurate anti-HIV assay applicable to serum/plasma. With few exceptions anti-HIV 1 positive specimens are reactive only on the second day and anti-HIV 2 positive specimens only on the third day. It thus discriminates anti-HIV 2 from anti-HIV 1, probably through depletion of cross-reacting antibodies by the reaction with the particles initially added. (C) 1995 Wiley-Liss, Inc.