PIXE MICROANALYSIS OF TRACE-ELEMENTS OF THE NORMOXIC, ISCHEMIC AND REPERFUSED RAT-HEART

被引：5

作者：

VERHOEF, BAW

FREDERIK, PM

BOMANS, PHH

VANDERVUSSE, GJ

MUTSAERS, PHA

DELEPPER, ECP

DEVOIGT, MJA

机构：

[1] UNIV LIMBURG, CARDIOVASC RES INST MAASTRICHT, DEPT PATHOL, EM UNIT, 6200 MD MAASTRICHT, NETHERLANDS

[2] EINDHOVEN UNIV TECHNOL, DEPT PHYS, CYCLOTRON LAB, 5600 MB EINDHOVEN, NETHERLANDS

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS | 1995年 / 104卷 / 1-4期

关键词：

D O I：

10.1016/0168-583X(95)00420-3

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Via PIXE with a 3 x 3 mu m(2) proton microbeam spatial distributions of Na, Mg, P, S, K, Ca and Fe were measured in 50 x 50 mu m(2) areas of rat heart, sliced into 10-15 mu m thick cryosections. The hearts were subjected to normal perfusion, ischemia and reperfusion. Substantial changes were found in tissue after 40 min of reperfusion, where locally elevated Ca levels and decreased K levels were observed. For assessment of artefacts due to sample preparation and handling also electron microscopy was used.

引用

页码：311 / 316

页数：6

共 14 条

[1] COPPER BLOCK METHOD FOR FREEZING NON-CRYOPROTECTED TISSUE TO PRODUCE ICE-CRYSTAL-FREE REGIONS FOR ELECTRON-MICROSCOPY .1. EVALUATION USING FREEZE-SUBSTITUTION [J].