PERFUSION OF FROGS SPINAL CORD AS A CONVENIENT METHOD FOR NEUROPHARMACOLOGICAL STUDIES

The isolated spinal cord of the bull frog was perfused through the anterior spinal artery. Under physiological conditions, the tissue survived for several hours and the spike potential of motorneurons, reflexes and ventral or dorsal root potentials remained constant during this time. This type of perfusion has the following advantages: it is simple to handle, the ionic environment of the neurons is easily controlled and small amounts of substances or substances which are weak in action can be tested. © 1969.