THE ALPHA-1-BETA-1 HETERODIMER OF ATP SYNTHASE

被引：16

作者：

OHTA, S

HARADA, M

ITO, Y

KOBAYASHI, Y

SONE, N

KAGAWA, Y

机构：

[1] JICHI MED SCH,DEPT BIOCHEM,MINAMI KAWACHI,TOCHIGI 32904,JAPAN

[2] UNIV TOKYO,INST SOLID STATE PHYS,MINATO KU,TOKYO 106,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90821-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The α3β3 hexamer was reconstituted from the α and β subunits of TF1 portion of ATP synthase of thermophilic bacterium (Kagawa et al. (1989) FEBS Lett. 249, 67). The α1β1 heterodimer of ATP synthase was isolated by high performance liquid chromatography (HPLC) of the α3β3 hexamer in the presence of AT(D)P-Mg. On polyacrylamide gel electrophoresis, both bands corresponding to the dimer and hexamer showed ATPase activity. The α1β1 dimer was dissociated into the equal amounts of the α and β monomers by sodium dodecyl sulfate. The α and β monomers were practically inactive. The α2 and β2 homodimers were not detected by electrophoresis and HPLC. © 1990.

引用

页码：1258 / 1263

页数：6

共 19 条

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