AFFINITY CROSS-FLOW FILTRATION - SOME NEW ASPECTS

被引：19

作者：

HERAK, DC

MERRILL, EW

机构：

[1] Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts

来源：

BIOTECHNOLOGY PROGRESS | 1990年 / 6卷 / 01期

关键词：

D O I：

10.1021/bp00001a005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A variety of affinity cross‐flow filtration (ACFF) experiments were conducted to evaluate the technique as a means of effectively separating biomolecules. Agarose particles, which contained a specific affinity ligand for the targeted protein, were used as “affinity escorts” in the ACFF process. Both conventional agarose particles (40–150; μm) and small agarose particles (Superose, 11–15 μm.) were used as the basis for affinity escorts. Batch ACFF washing experiments were conducted in both constant‐volume and volume‐reduction modes and compared with model predictions. The different affinity ligand‐adsorbate systems studied include Cibacron Blue and human serum albumin, Cibacron Blue and lysozyme, protein A and immunoglobulin G, and concanavalin A and horseradish peroxidase. Copyright © 1990 American Institute of Chemical Engineers (AIChE)

引用

页码：33 / 40

页数：8

共 19 条

[1] Dwyer J.L., Bio/Technology, 2, (1983)
[2] Mattiasson B., Ling T.G., Membrane Separations in Biotechnology, (1986)
[3] Luong J.H.T., Nguyen A.L., Male K.B., Affinity Cross–Flow Filtration for Purifying Biomolecules, Bio/Technology, 5, (1987)
[4] Pungor E., Afeyan N.B., Gordon N.F., Cooney C.L., Continuous Affinity–Recycle Extraction: A Novel Protein Separation Technique, Bio/Technology, 5, (1987)
[5] Herak D.C., Merrill E.W., Biotechnol. Prog., 5, (1989)
[6] Gutman R.G., Membrane Filtration: The Technology of Pressure‐Driven Crossflow Processes, (1987)
[7] Arve B.H., Liapis A.I., AIChE J., 33, (1987)
[8] Arve B.H., Liapis A.I., Modeling and analysis of elution stage of biospecific adsorption in finite bath, Biotechnology and Bioengineering, 31, (1988)
[9] Arnold F.H., Blanch H.W., Wilke C.R., Analysis of affinity separations, The Chemical Engineering Journal, 30, (1985)
[10] Carleysmith S.W., Eames M.B.L., Lilly M.D., Biotechnol. Bioeng., 22, (1980)

← 1 2 →