INVESTIGATION OF RED-BLOOD-CELL FRACTIONATION BY GRAVITATIONAL FIELD-FLOW FRACTIONATION

被引：32

作者：

URBANKOVA, E ^{[1
]}

VACEK, A ^{[1
]}

NOVAKOVA, N ^{[1
]}

MATULIK, F ^{[1
]}

CHMELIK, J ^{[1
]}

机构：

[1] CZECHOSLOVAK ACAD SCI, INST BIOPHYS, CS-61265 BRNO, CZECHOSLOVAKIA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 583卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80341-M

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Gravitational field-flow fractionation is used for the separation of particles according to their sizes in the range 1-100 mum: larger particles elute before smaller ones. This phenomenon can be explained as a result of the steric exclusion of the particles from the vicinity of the channel walls, and/or hydrodynamic effects supposedly associated with the inertia of the liquid. The method was used for the investigation of red blood cells. The dependence of the retention ratio on the flow-rate. sample volume. concentration of blood and relaxation time was studied. Analysis of fifteen individual fractions by Coulter counter and reinjection of three other fractions were studied in order to verify fractionation of red blood cells.

引用

页码：27 / 34

页数：8

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