THE CLONING AND SEQUENCING OF THE GENES ENCODING PHYTASE (PHY) AND PH 2.5-OPTIMUM ACID-PHOSPHATASE (APH) FROM ASPERGILLUS-NIGER VAR AWAMORI

被引：77

作者：

PIDDINGTON, CS

HOUSTON, CS

PALOHEIMO, M

CANTRELL, M

MIETTINENOINONEN, A

NEVALAINEN, H

RAMBOSEK, J

机构：

[1] AIKO LTD, RES LABS, SF-00101 HELSINKI, FINLAND

[2] PANLABS INC, 11804 N CREEK PKWY S, BOTHELL, WA 98011 USA

来源：

GENE | 1993年 / 133卷 / 01期

关键词：

PHYTATE; INOSITOL HEXAPHOSPHATE; RECOMBINANT DNA; DEGENERATE OLIGODEOXYRIBONUCLEOTIDE; INTRON; CODON BIAS; OVEREXPRESSION; HOMOLOGY;

D O I：

10.1016/0378-1119(93)90224-Q

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The genes encoding phytase (EC 3.1.3.8) and pH 2.5-optimum acid phosphatase (EC 3.1.3.2) have been cloned and sequenced from Aspergillus niger var. awamori. The translated nucleotide sequences yielded polypeptides of 467 and 479 amino acids (aa) for phytase and acid phosphatase, respectively. The genes were isolated using oligodeoxyribonucleotide probes based on the aa sequences of the purified proteins. Recombinant A. niger var. awamori strains carrying additional copies of the gene sequences demonstrated elevated enzyme activities.

引用

页码：55 / 62

页数：8

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