ISOLATION AND CHARACTERIZATION OF 2 SUBSPECIES OF LP(A), ONE CONTAINING APO-E AND ONE FREE OF APO-E

被引：33

作者：

BARD, JM ^{[1
]}

DELATTRELESTAVEL, S ^{[1
]}

CLAVEY, V ^{[1
]}

PONT, P ^{[1
]}

DERUDAS, B ^{[1
]}

PARRA, HJ ^{[1
]}

FRUCHART, JC ^{[1
]}

机构：

[1] INSERM, U325, F-59019 LILLE, FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1127卷 / 02期

关键词：

APO-E; APOLIPOPROTEIN (A); LIPOPROTEIN BINDING;

D O I：

10.1016/0005-2760(92)90267-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipoprotein Lp(a) was isolated by immunoaffinity chromatography using anti apolipoprotein B and anti apolipoprotein (a) immunosorbents. Besides apolipoproteins (a) and B, this fraction was shown to contain apolipoproteins C and E. Therefore, it was decided to further purify this crude Lp(a) into particles containing apolipoprotein E and particles free of apo E, using chromatography with an anti apolipoprotein E immunosorbent. Lp(a), free of apolipoprotein E was cholesterol ester rich and triacylglycerol poor and was found mainly in the LDL size range. In contrast, Lp(a) containing apolipoprotein E was triacylglycerol rich and was distributed mainly in the VLDL and IDL size range. Binding of these two fractions, one containing apo E and one free of it, to the apo B/E receptor of HeLa cells was studied. Both fractions bound to the receptor but the one containing apo E had a better affinity than the one free of apo E. Further studies are needed to identify the clinical importance of these two different entities.

引用

页码：124 / 130

页数：7

共 41 条

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