SEPARATION OF IMIDAZOLE AND ITS DERIVATIVES BY CAPILLARY ELECTROPHORESIS

The use of capillary electrophoresis (CE) for the separation of imidazole and its derivatives is reported. In the first part of the investigation, efforts were focused on method development, where the effects of buffer pH, types of buffer modifiers and modifier concentration on the separation of these compounds were examined. The second part was focused on method validation, where the ruggedness, selectivity, limits of detection and linearity were investigated. In the final part, the CE method was applied to commercial-grade imidazole. A comparison of the results obtained using the CE system was made with those obtained by HPLC. Good correlation between the two sets of results was obtained and superior efficiencies and better peak shapes for most of the imidazoles were also achieved using the CE system.