2 TRANSFER RNA-BINDING SITES IN ADDITION TO A-SITE AND P-SITE ON EUKARYOTIC RIBOSOMES

被引:22
作者
RODNINA, MV [1 ]
WINTERMEYER, W [1 ]
机构
[1] UNIV WITTEN HERDECKE,INST MOLEK BIOL,STOCKUMER STR 10,W-5810 WITTEN,GERMANY
关键词
80S RIBOSOMES; RIBOSOMAL E-SITE; RIBOSOMAL TRANSLOCATION; RIBOSOMAL TRANSFER RNA-BINDING SITES; TOPOLOGY OF TRANSFER RNA RIBOSOME INTERACTION;
D O I
10.1016/0022-2836(92)90834-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The interaction of tRNA with 80 S ribosomes from rabbit liver was studied using biochemical as well as fluorescence techniques. Besides the canonical A and P sites, two additional sites were found which specifically bind deacylated tRNA. One of the sites is analogous to the E site of prokaryotic ribosomes, in that binding of tRNA is labile, does not depend on codon-anticodon interaction, does not protect the anticodon loop from solvent access, and requires the presence of the 3′-terminal adenosine of the tRNA. In contrast, the stability of the tRNA complex with the second site (S site) is high. tRNA binding to the S site is also codon-independent; nevertheless, the anticodon loop is shielded from solvent access. Removal of the 3′-terminal adenosine decreases the affinity of tRNAPhe for the S site approximately 50-fold. tRNAPhe is retained at the S site during translocation and through poly(Phe) synthesis. Thus, the S site does not seem to be an intermediate site for the tRNA during the elongation cycle. Rather, the tRNA bound to the S site may allosterically modulate the function of the ribosome. © 1992.
引用
收藏
页码:450 / 459
页数:10
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