A COLORIMETRIC ASSAY FOR AMINO NITROGEN IN SMALL VOLUMES OF BLOOD - REACTION WITH BETA-NAPHTHOQUINONE SULFONATE

被引：36

作者：

EVANS, PC

FFOLLIOTTPOWELL, FM

HARDING, JE

机构：

[1] Research Centre for Developmental Medicine and Biology, University of Auckland, Auckland

来源：

ANALYTICAL BIOCHEMISTRY | 1993年 / 208卷 / 02期

关键词：

D O I：

10.1006/abio.1993.1056

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay has been developed for the colorimetric estimation of total amino acids in small volumes (100 μl) of whole blood. The procedure utilizes the reaction of amino nitrogen with β-naphthoquinone sulfonate. It represents a substantial improvement on previously published methods by decreasing reagent volumes and accurately specifying and maintaining pH conditions for the reaction itself (9.2-9.4) and for removal of excess reagent prior to measurement of the resulting complex (2.2-2.4). Ammonia is the only compound shown to significantly interfere with the assay but this is not a problem at physiological concentrations. © 1994 Academic Press, Inc. All rights reserved.

引用

页码：334 / 337

页数：4

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