KERATINOCYTE GROWTH-FACTOR AS A MITOGEN FOR PRIMARY CULTURE OF RAT HEPATOCYTES

被引：24

作者：

ITOH, T

SUZUKI, M

MITSUI, Y

机构：

[1] NATL INST BIOSCI & HUMAN TECHNOL, CELL BIOL LAB, HIGASHI 1-1, TSUKUBA, IBARAKI 305, JAPAN

[2] MAYEKAWA MFG CO LTD, ADV TECHNOL LAB, MORIYA, IBARAKI 30201, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 192卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1517

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In a series of our studies on identification of novel mitogens for hepatocytes, keratinocyte growth factor (KGF, FGF-7) is found to have a stimulatory effect on DNA synthesis of adult rat hepatocytes in primary culture. Stimulation of DNA synthesis with KGF was seen to be dose dependent with its maximum effect at 15-50ng/ml. Studies on nuclear labeling with BrdU confirmed the mitogenic effect of KGF on rat hepatocytes. The mitogenic effect of KGF was greater than those of EGF, FGF-1 and comparable to that of HGF. Further, it exhibited additive mitogenic effect when present along with the optimum concentration of HGF, EGF or FGF-1. Thus, it is concluded that KGF is a potent mitogen for rat hepatocytes in vitro. © 1993 Academic Press, Inc.

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页码：1011 / 1015

页数：5

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