INDUCTION EXPERIMENTS WITH ADENOVIRUS AND POLYOMA VIRUS TRANSFORMED CELL LINES

Techniques used successfully in inducing SV40‐transformed cells to produce infectious SV40 were employed in an attempt to induce cells transformed by other oncogenic DNA viruses, adenoviruses 2 and 12 and polyoma virus. These techniques included cocultivation and fusion of transformed cells with indicator cells, and mitomycin C treatment of these heterokaryons and of transformed cells. In addition, prior to induction experiments, some cell lines were passaged through animals or in the presence of BUdR in an effort to enhance their inducibility. All attempts to induce these transformed cells were unsuccessful. It is concluded from this study that the polyoma and adenovirus genomes, if present in their entirety in cells transformed with these viruses, are not as reactivable as is the SV40 genome in SV40‐transformed cells. Copyright © 1969 Wiley‐Liss, Inc., A Wiley Company