A RAPID AND REPRODUCIBLE METHOD FOR OBTAINING YEAST PROTOPLASTS

被引：16

作者：

SCHWENCKE, J

GONZALEZ, G

FARIAS, G

机构：

[1] Instituto de Química—Departamento de Ciencias, Universidad de Chile, Valparaíso

来源：

JOURNAL OF THE INSTITUTE OF BREWING | 1969年 / 75卷 / 01期

关键词：

D O I：

10.1002/j.2050-0416.1969.tb03176.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Protoplasts were prepared from exponentially growing yeast cells with a lytic preparation of Streptomyces WL‐6. The following yeast strains were studied: Saccharomyces chevalieri, Candida albicans, Schizosaccharomyces pombe and Sacch. cerevisiae. True protoplasts were obtained in 100% yield after 15 min. incubation with the lytic preparation from Sacch. chevalieri and after 90 min. from Sacch. cerevisiae. For Schizosacch. pombe 10% of the cells were converted into protoplasts in the best conditions. Only spheroplasts were obtained from C. albicans. 1969 The Institute of Brewing & Distilling

引用

页码：15 / +

页数：1

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