GENETIC STUDIES OF RIBOSOMAL PROTEINS IN ESCHERICHIA COLI .I. MUTANTS AND STRAINS HAVING 30S RIBOSOMAL SUBUNIT WITH ALTERED PROTEIN COMPONENTS

Two 30s ribosomal protein components, 30-4K and 30-7K, from E. coli K12 strain were clearly distinguished on the CMC column chromatogram from the corresponding protein components, 30-4B and 30-7B, from B strain. The 30-7K component was shown to correspond to the K-character. A mutant strain of K12, W3637, had an altered 30s ribosomal protein component, 30-9W3637. The characters of 30-4K, 30-7K, 30-9W3637 and strr were found to be cotransduced from W3637 to B strain by Plke phage in 16 out of 20 strr transductants. The 30-9W3637 and 30-4K components were separated from strr in 4 strr transductants. These results indicate that (1) neither 30-4 nor 30-9 is the protein whose mutational change leads to strr, and (2) the genes specifying the 30s ribosomal proteins, 30-4, 30-7, 30-9 and str are linked on the chromosome. © 1969 Springer-Verlag.