EFFECT OF OSMOLARITY ON IN-VITRO DEVELOPMENT OF RABBIT EMBRYOS IN A CHEMICALLY DEFINED MEDIUM

被引：32

作者：

NAGLEE, DL

MAURER, RR

FOOTE, RH

机构：

[1] Department of Animal Science, Cornell University, Ithaca

来源：

EXPERIMENTAL CELL RESEARCH | 1969年 / 58卷 / 2-3期

关键词：

D O I：

10.1016/0014-4827(69)90513-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Rabbit embryos were cultured from the 2- to 4-cell stage in a modified Ham's F-10 defined medium prepared so as to give calculated osmolarities of 230, 250, 270, 290, 310 and 330 mOsm. After 120 h in culture, the number of embryos reaching the blastocyst stage in the 270 mOsm medium was significantly (P < 0.05) higher than that at 310 and 330 mOsm. The number of blastocysts which had enlarged and partially or completely escaped from the zona pellucida at 270 mOsm was significantly higher than at 230, 310, and 330 mOsm (P < 0.01) and 250 mOsm (P < 0.05). © 1969.

引用

页码：331 / &

共 12 条

[1]

BRINSTER RL, 1965, J REPROD FERTIL, V10, P227

[2] STUDIES ON DEVELOPMENT OF MOUSE EMBRYOS IN VITRO .I. EFFECT OF OSMOLARITY AND HYDROGEN ION CONCENTRATION [J].