EXPRESSION OF TYROSINASE IN VEGETATIVE CULTURES OF NEUROSPORA-CRASSA TRANSFORMED WITH A METALLOTHIONEIN PROMOTER PROTYROSINASE FUSION GENE

被引:13
作者
KUPPER, U [1 ]
LINDEN, M [1 ]
CAO, K [1 ]
LERCH, K [1 ]
机构
[1] UNIV ZURICH,INST BIOCHEM,CH-8057 ZURICH,SWITZERLAND
关键词
Expression system; Metallothionein promoter; N; crassa; Tyrosinase;
D O I
10.1007/BF00318214
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is only expressed in situations of starvation or sexual differentiation. Complete integration of a 400 bp metallothionein promoter-fragment leads to constitutive expression of protyrosinase, whereas a 3.6 kb promoter-fragment conferred copper inducibility on the reporter gene in four transformants. A transformant with high constitutive tyrosinase levels was able to produce melanin on complete medium agar plates supplemented with 1 mg/ml L-tyrosine. © 1990 Springer-Verlag.
引用
收藏
页码:331 / 335
页数:5
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