ELECTROPHORETIC METHOD FOR THE DETECTION OF HUMAN RED-CELL ACETYLCHOLINESTERASE

被引：6

作者：

RAVAZZOLO, R

BRUZZONE, G

GARRE, C

AJMAR, F

机构：

[1] Department of Haematology, ISMI, University of Genoa

来源：

BIOCHEMICAL GENETICS | 1979年 / 17卷 / 11-1期

关键词：

acetylcholinesterase; erythrocyte membrane; screening procedure;

D O I：

10.1007/BF00504351

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An electrophoretic method to demonstrate human red cell membrane acetylcholinesterase is described. The method, performed with crude Triton lysates of red cells on a flat-bed polyacrylamide gel, is sufficiently simple to be suitable for a population survey. © 1979 Plenum Publishing Corporation.

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收藏

页码：1145 / 1149

页数：5

相关论文

共 12 条

[1] GENETIC-VARIATION IN HUMAN ERYTHROCYTE ACETYLCHOLINESTERASE [J].

COATES, PM ;

SIMPSON, NE .

SCIENCE, 1972, 175 (4029) :1466-&

[2]

DANON D, 1964, J LAB CLIN MED, V64, P668

[3]

Dewald B, 1974, Methods Enzymol, V32, P82

[4] A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].

ELLMAN, GL ;

COURTNEY, KD ;

ANDRES, V ;

FEATHERSTONE, RM .

BIOCHEMICAL PHARMACOLOGY, 1961, 7 (02) :88-&

[5]

Hanahan D J, 1974, Methods Enzymol, V31, P168

[6]

HARRIS H, 1976, HDB ENZYME ELECTROPH, P9

[7] REVIEW - HUMAN ERYTHROCYTE ACETYLCHOLINESTERASE [J].

HERZ, F ;

KAPLAN, E .

PEDIATRIC RESEARCH, 1973, 7 (04) :204-214

[8] DIRECT-COLORING THIOCHOLINE METHOD FOR CHOLINESTERASES [J].

KARNOVSKY, MJ ;

ROOTS, L .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1964, 12 (03) :219-&

[9] STUDIES ON CHARACTERIZATION OF HUMAN ERYTHROCYTE ACETYLCHOLINESTERASE AND ITS INTERACTION WITH ANTIBODIES [J].

NIDAY, E ;

WANG, CS ;

ALAUPOVIC, P .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 469 (02) :180-193

[10] MULTIPLE MOLECULAR-FORMS OF ACETYLCHOLINESTERASE FROM HUMAN ERYTHROCYTE-MEMBRANES - INTERCONVERSION AND SUBUNIT COMPOSITION OF FORMS SEPARATED BY DENSITY GRADIENT CENTRIFUGATION IN A ZONAL ROTOR [J].

OTT, P ;

BRODBECK, U .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 88 (01) :119-125