Procedures for the measurement with glass electrodes of ion-exchange processes involving Na+, K+, and H+ have been described. Features characteristic of the transfers of Na+ and K+ during rewarming of the precooled rat tail artery were shown to illustrate the application of the technique to short- and long-term metabolic studies. The measurement of the exchange of Na+ for H+ during perfusion of an artery with a Na+-free medium was used as illustration for the application of the method to study of ion-exchange processes. © 1969.