RAT HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE CDNA CLONING AND SEQUENCE-ANALYSIS

被引：17

作者：

CHIAVEROTTI, TA

BATTULA, N

MONNAT, RJ

机构：

[1] UNIV WASHINGTON,DEPT PATHOL,SM-30,SEATTLE,WA 98195

[2] NCI,EXPTL CARCINOGENESIS LAB,BETHESDA,MD 20892

来源：

GENOMICS | 1991年 / 11卷 / 04期

关键词：

D O I：

10.1016/0888-7543(91)90046-H

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have determined the nucleotide sequence of the rat hprt (hypoxanthine phosphoribosyltransferase; EC 2.4.2.8.) mRNA coding region and of adjacent, untranslated 5′ and 3′ mRNA, and we have designed an oligonucleotide primer pair for efficient PCR amplification of the rat hprt coding region. These sequence data and rat-specific primer pair will aid workers interested in coupling well-developed rat toxicologic and carcinogenicity bioassays with quantitative and molecular analyses of somatic mutation induction in rat cells in vivo and in vitro. © 1991.

引用

页码：1158 / 1160

页数：3

共 9 条

[1] MOLECULAR-BASIS OF HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE DEFICIENCY IN 10 SUBJECTS DETERMINED BY DIRECT SEQUENCING OF AMPLIFIED TRANSCRIPTS [J].