LOCALIZATION OF ISOPENICILLIN N SYNTHASE IN PENICILLIUM-CHRYSOGENUM PQ-96

被引：15

作者：

KURZATKOWSKI, W

PALISSA, H

VANLIEMPT, H

VONDOHREN, H

KLEINKAUF, H

WOLF, WP

KURYLOWICZ, W

机构：

[1] INST BIOCHEM & MOLEC BIOL,W-1000 BERLIN 10,GERMANY

[2] ROBERT KOCH INST,FED HLTH OFF,W-1000 BERLIN 65,GERMANY

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1991年 / 35卷 / 04期

关键词：

D O I：

10.1007/BF00169760

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

For immunoelectron microscopic localization of isopenicillin N synthase (IPNS), glutaraldehyde-fixed mycelium of Penicillium chrysogenum PQ-96 was dehydrated by progressive lowering of the temperature and embedded into Lowicryl K4M at -35-degrees-C. This procedure resulted in good structural preservation such that the method of on-section labelling using antibody to IPNS from Cephalosporium acremonium CO 278 with the indirect antibody-gold technique could be successfully applied. By this method IPNS was localized in vesicular compartments belonging probably to the Golgi body and in the cell wall.

引用

页码：517 / 520

页数：4

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