LICL PROMOTES SURVIVAL OF GABAERGIC NEURONS FROM CEREBELLUM AND CEREBRAL-CORTEX - LICL INDUCES SURVIVAL OF GABAERGIC NEURONS

被引：19

作者：

VOLONTE, C

CIOTTI, MT

MERLO, D

机构：

[1] Institute of Neurobiology, CNR, 00137 Rome

来源：

NEUROSCIENCE LETTERS | 1994年 / 172卷 / 1-2期

关键词：

RAT; PRIMARY CULTURE; GRANULE NEURON;

D O I：

10.1016/0304-3940(94)90649-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Previous studies showed that LiCl promotes short-term survival of PC12 cells after NGF or serum deprivation. In the present work, we investigate the survival effect of lithium on cerebellar granule primary cultures. While the total population of cerebellar neurons, mainly granule cells, showed only a short-term survival (about 20 h) in the presence of LiCl, the survival of 65-100% of the GABAergic interneurons originating from cerebellum and cerebral cortex at two different developmental stages was prolonged by 1-2 weeks. Optimal activity was elicited between 5 and 7 mM LiCl. The action of lithium required the presence of serum and persisted also after medium renewal. By direct visual inspection, LiCl promoted neuronal survival without apparently altering the morphological differentiation of the cells. Our studies thereby suggest a means to obtaining enriched populations of GABAergic neurons.

引用

页码：6 / 10

页数：5

共 22 条

[1]

ALOISI F, 1985, J NEUROSCI, V5, P2001

[2] STIMULATION OF THE N-METHYL-D-ASPARTATE RECEPTOR HAS A TROPHIC EFFECT ON DIFFERENTIATING CEREBELLAR GRANULE CELLS [J].