RAPID PURIFICATION METHOD FOR HUMAN RECOMBINANT TUMOR-NECROSIS-FACTOR-ALPHA

被引：6

作者：

PAQUET, A ^{[1
]}

LEVESQUE, A ^{[1
]}

PAGE, M ^{[1
]}

机构：

[1] UNIV LAVAL,FAC MED,DEPT BIOCHEM,ST FOY G1K 7P4,PQ,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 667卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)89060-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human recombinant tumor necrosis factor alpha was purified in a single step to about 95% purity from Escherichia coli lysate by chromatography on hydroxyapatite. The last traces of contaminants were removed by fast protein liquid chromatography on a Mono Q column. The final product was found to be pure by gel electrophoresis with silver staining. A molecular mass of approximately 17 000 and a specific activity of 4.3 . 10(6) U/mg after a single purification step were found.

引用

页码：125 / 130

页数：6

共 16 条

[1]

AGGARWAL BB, 1985, J BIOL CHEM, V260, P2345

[2]

AGGARWAL BB, 1985, METHOD ENZYMOL, V116, P443

[3]

ARAKAWA T, 1987, J BIOL CHEM, V262, P7484

[4] THE MOLECULAR ACTION OF TUMOR-NECROSIS-FACTOR-ALPHA [J].

CAMUSSI, G ;

ALBANO, E ;

TETTA, C ;

BUSSOLINO, F .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 202 (01) :3-14

[5] ENDOTOXIN-INDUCED SERUM FACTOR THAT CAUSES NECROSIS OF TUMORS [J].

CARSWELL, EA ;

OLD, LJ ;

KASSEL, RL ;

GREEN, S ;

FIORE, N ;

WILLIAMSON, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (09) :3666-3670

[6]

DAVIS JM, 1987, BIOCHEMISTRY-US, V26, P1326

[7]

GLAZER AN, 1975, CHEM MODIFICATION PR, P23

[8]

HUGLI TE, 1972, J BIOL CHEM, V247, P2828

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] STRUCTURE OF TUMOR NECROSIS FACTOR BY X-RAY SOLUTION SCATTERING AND PRELIMINARY STUDIES BY SINGLE-CRYSTAL X-RAY-DIFFRACTION [J].

LEWITBENTLEY, A ;

FOURME, R ;

KAHN, R ;

PRANGE, T ;

VACHETTE, P ;

TAVERNIER, J ;

HAUQUIER, G ;

FIERS, W .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 199 (02) :389-392

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