CORRELATION FREE-SOLUTION CAPILLARY ELECTROPHORESIS MIGRATION TIMES OF SMALL PEPTIDES WITH PHYSICOCHEMICAL PROPERTIES

被引：18

作者：

CHEN, N

WANG, L

ZHANG, YK

机构：

[1] National Chromatographic R. and A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian

来源：

CHROMATOGRAPHIA | 1993年 / 37卷 / 7-8期

关键词：

CAPILLARY ELECTROPHORESIS; MIGRATION TIME PREDICTION; SMALL PEPTIDES;

D O I：

10.1007/BF02272260

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of small peptides containing varying degree of charge and size was used to study the effects of physicochemical properties on migration in free-solution capillary electrophoresis (FSCE). A semiempirical relationship between migration time under acidic conditions and the square root of molecular weight divided by the quantity of the number of the positively ionizable groups has been established. The ionization of the carboxyl terminal group in the polypeptides is negligible under acidic conditions. The relationship developed from this work has been used for the prediction of migration parameters in free solution capillary electrophoresis.

引用

页码：429 / 432

页数：4

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