RDNA TARGETED OLIGONUCLEOTIDE PRIMERS FOR THE IDENTIFICATION OF PATHOGENIC YEASTS IN A POLYMERASE CHAIN-REACTION

被引：21

作者：

FELL, JW ^{[1
]}

机构：

[1] UNIV MIAMI, ROSENSTIEL SCH MARINE & ATMOSPHER SCI, MIAMI, FL 33149 USA

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1995年 / 14卷 / 06期

关键词：

CRYPTOCOCCUS NEOFORMANS; RHODOTORULA MUCILAGINOSA; TRICHOSPORON CUTANEUM; BASIDIOMYCETES; MOLECULAR TAXONOMY; POLYMERASE CHAIN REACTION;

D O I：

10.1007/BF01573961

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Species-specific oligonucleotide primers were designed for PCR identification of the basidiomycetous yeasts Cryptococcus neoformans. Trichosporon cutaneum and Rhodotorula mucilaginosa. The procedure uses standard PCR components including DNA from the test species and three primers: two universal external (upstream and downstream) limiting primers and a species-specific internal primer. Species identification requires the formation of a species-specific rDNA nucleotide segment that is significantly smaller (similar to 200 bp) than a non-target segment (similar to 600 bp). The procedure can be used to identify yeasts from single and mixed populations.

引用

页码：475 / 477

页数：3

共 10 条

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