A NEW DETERMINATION OF OXIDIZED AND REDUCED NICOTINAMIDE-ADENINE DINUCLEOTIDE IN TISSUES

被引：10

作者：

KANEKO, T

FIELD, JB

机构：

[1] Clinical Research Unit, University of Pittsburgh School of Medicine, Pittsburgh

[2] Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 29卷 / 02期

关键词：

D O I：

10.1016/0003-2697(69)90302-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and simple method for measuring NAD and NADH in the range of 0 to 200 μμmoles is described. The assay is based on the enzymic cycling of NAD and NADH and the sequential conversion of lactate-1-14C to 14CO2 in the presence of NAD or NADH, Mg++, thiamine pyrophosphate, α-ketoglutarate, glutamic dehydrogenase, lactate dehydrogenase, and pyruvate dehydrogenase. Recovery of internal standard added to the tissue extract was approximately 100% and the assay of two different amounts of tissue resulted in equivalent concentrations of NAD or NADH per gram tissue. © 1969.

引用

页码：193 / &

共 17 条

[1] DETERMINATION OF THE REDUCED AND OXIDIZED PYRIDINE NUCLEOTIDES IN ANIMAL TISSUES [J].