REGULATORY REGION OF MS2-PHAGE RNA REPLICASE CISTRON .3. CHARACTERIZATION OF FRAGMENTS RESULTING FROM S1 NUCLEASE DIGESTION

被引:17
作者
JANSONE, I
BERZIN, V
GRIBANOV, V
GREN, EJ
机构
[1] Laboratory of Nucleic Acid Chemistry, Institute of Organic Synthesis, Latvian Academy of Sciences
关键词
D O I
10.1093/nar/6.5.1747
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The functionally active fragments MS2 R(-53←6) and MS2 R(-53←3) comprising the regulatory region for the replicase cistron have been isolated from MS2 RNA-coat protein complex following TI RNase digestion. In order to obtain shorter fragments, active in coat protein binding and initiation of translation, MS2 R(-53←6) was cleaved with S1 nuclease. The results indicate that Sl nuclease attacks the most susceptible loop regions of the two hairpin helices of MS2 R(-53←6). Amon the three fragments which have been isolated, only W 2 R(-35/33←6) containing the intact hairpin (b) region with initiation codon AUG is active in the coat protein binding. Functional activity exerted by another polynucleotide MS2 R(-17←6) supports the assumption that specific binding with the coat protein is determined by the hairpin (b) region prior to the replicase cistron. © 1979 Information Retrieval Limited.
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页码:1747 / 1760
页数:14
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