INTER-MOLECULAR TRANSGLYCOSYLATION OF CYCLODEXTRIN GLYCOSYLTRANSFERASE TO D-GALACTOSE

Intermolecular transglycosylation of cyclodextrin glycosyltransferase from B. megaterium strain No. 5 was examined in the reaction system containing D-galactose and soluble starch. The yield of transfer products to D-galactose were 2 to 3 per cent of those to D-glucose. Four kinds of transfer products to D-galactose as the acceptor were obtained. These compounds were identified to be 1-O-a-D-glucosyl-D-galactose, 3-O-a-D-glucosyl-D-galactose, 2-O-a-D- glucosyl-D-galactose and 4-O-a-D-glucosyl-D-galactose. The transglycosylation to D-galactose was shown to occur at various hydroxyl groups of the acceptor; at C1-, C3- and C2-(C4-) hydroxyl group in the proportion of 26: 10: 1. © 1979, by the Agricultural Chemical Society of Japan.