CD16 AND CR3 RECEPTORS DISTINGUISH BETWEEN THE 2 MECHANISMS OF TUMOR-CYTOTOXICITY IN NEUTROPHILS

被引:11
作者
GAVIOLI, R
SPISANI, S
GIULIANI, AL
COSULICH, E
RISSO, A
TRANIELLO, S
机构
[1] UNIV FERRARA,IST CHIM BIOL,VIA L BORSARI 46,I-44100 FERRARA,ITALY
[2] IST NAZL RIC CANC,GENOA,ITALY
关键词
D O I
10.1111/j.1365-2141.1991.tb04518.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Previous studies have suggested that phorbol ester-activated neutrophils kill both antibody non-coated and antibody-coated K562 target cells. In this report the contribution of the receptors Fc-gamma-III (CD16) and CR3 (CD11b/CD18) in the lytic process was investigated. In neutrophils CD16 and CR3 are up-regulated by the phorbol ester up to 4 and 10 times, respectively. As expected, lysis of non-immunized K562 targets is not affected by the treatment of neutrophils with anti CD16, AB8.28, whereas lysis of immunized targets is decreased by 50%. In addition, the interaction of CD16 and AB8.28 induces calcium mobilization and increases granule secretion. Surprisingly, the simultaneous binding of AB8.28 and anti-CR3 OKM1 to neutrophils completely abolishes the lysis of antibody-coated targets. Unlike CD16, CR'3 does not possess a functional role and binding of OKM1 to CR3 does not affect cytotoxicity of immunized K562 targets, but it blocks lysis of non-coated target almost completely, indicating a function as adhesion protein for CR3. These studies demonstrate a distinct role of CD16 and CR3 in mediating antibody-dependent and antibody-independent cellular cytotoxicity, respectively.
引用
收藏
页码:170 / 176
页数:7
相关论文
共 33 条
[1]   FUNCTIONAL-CHARACTERIZATION OF AN ANTIGEN INVOLVED IN AN EARLY STEP OF T-CELL ACTIVATION [J].
COSULICH, ME ;
RUBARTELLI, A ;
RISSO, A ;
COZZOLINO, F ;
BARGELLESI, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (12) :4205-4209
[2]  
DALLEGRI F, 1985, IMMUNOLOGY, V55, P639
[3]  
ERBE DU, 1990, MOL IMMUNOL, V26, P57
[4]   CYTO-TOXICITY MEDIATED BY HUMAN FC-RECEPTORS FOR IGG [J].
FANGER, MW ;
SHEN, L ;
GRAZIANO, RF ;
GUYRE, PM .
IMMUNOLOGY TODAY, 1989, 10 (03) :92-99
[5]   INTERLEUKIN-3 AND PHORBOL ESTER STIMULATE TYROSINE PHOSPHORYLATION OF OVERLAPPING SUBSTRATE PROTEINS [J].
FERRIS, DK ;
WILLETTEBROWN, J ;
MARTENSEN, T ;
FARRAR, WL .
FEBS LETTERS, 1989, 246 (1-2) :153-158
[6]  
GAVIOLI R, 1990, CLIN EXP IMMUNOL, V80, P247
[7]   PROTEIN-KINASE-C MEDIATES HUMAN NEUTROPHIL CYTOTOXICITY [J].
GAVIOLI, R ;
SPISANI, S ;
GIULIANI, A ;
TRANIELLO, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 148 (03) :1290-1294
[8]  
GRAZIANO RF, 1987, J IMMUNOL, V139, P3536
[9]  
GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440
[10]   THE EXPRESSION OF CR-1 AND CR3 ON NON-MODULATED AND MODULATED GRANULOCYTES OF HEALTHY BLOOD-DONORS AS MEASURED BY FLOW CYTOFLUOROMETRY [J].
HED, J ;
BERG, O ;
FORSLID, J ;
HALLDEN, G ;
LARKARAFNER, G .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1988, 28 (03) :339-344