LARGE MUTAGENIC LESIONS ARE INDUCED BY PHOTODYNAMIC THERAPY IN MURINE L5178Y LYMPHOBLASTS

被引：21

作者：

DEAHL, JT ^{[1
]}

OLEINICK, NL ^{[1
]}

EVANS, HH ^{[1
]}

机构：

[1] CASE WESTERN RESERVE UNIV,DEPT RADIOL,DIV RADIAT BIOL,CLEVELAND,OH 44106

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1993年 / 58卷 / 02期

关键词：

D O I：

10.1111/j.1751-1097.1993.tb09558.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mutagenic lesions at the thymidine kinase locus (tk) in mouse lymphoma L5178Y (LY) cells treated with red light and either Photofrin (PF) or chloroaluminum phthalocyanine (AlPc) as the photosensitizer were compared in the relatively photodynamic therapy (PDT)-sensitive strain LY-R16 and the relatively resistant strains LY-S1 and LY-SR1. Southern blot analysis revealed that 92% (36/39) of the PDT-induced thymidine kinase (TK-/-) mutants of strains LY-R16 and LY-SR1 lost the entire active tk allele. (Strain LY-S1 lacks a known tk polymorphism and has not been analyzed for loss of the active tk allele.) A decrease in galactokinase (GK) activity in the TK-/- mutants has been taken as an indication that the mutagenic lesion extends from the tk gene to the closely linked galactokinase gene (gk). Using PF as the photosensitizer, GK activity was decreased in 45% of the LY-R16 mutants and in 22% of the LY-S1 and LY-SR1 mutants. With photoactivated AlPc, 59% of the TK-/- mutants of strains LY-S1 and LY-SR1 showed GK inactivation. (LY-R16 mutants were not analyzed because of the low LY-R16 mutant frequency induced by PDT with AlPc.) Thus, many of the TK-/- mutants of LY cells induced by PDT with either PF or AlPc harbor multilocus lesions.

引用

页码：259 / 264

页数：6

共 31 条

[1] INVOLVEMENT OF SINGLET OXYGEN IN CHLOROALUMINUM PHTHALOCYANINE TETRASULFONATE-MEDIATED PHOTOENHANCEMENT OF LIPID-PEROXIDATION IN RAT EPIDERMAL MICROSOMES [J].

AGARWAL, R ;

ATHAR, M ;

URBAN, SA ;

BICKERS, DR ;

MUKHTAR, H .

CANCER LETTERS, 1991, 56 (02) :125-129

[2] MOUSE LYMPHOMA CELLS WITH DIFFERENT RADIOSENSITIVITIES [J].

ALEXANDER, P .

NATURE, 1961, 192 (480) :572-&

[3] USE OF DNA PURIFIED INSITU FROM CELLS EMBEDDED IN AGAROSE PLUGS FOR THE MOLECULAR ANALYSIS OF TK-/- MUTANTS RECOVERED IN THE L5178Y TK+/-3.7.2C MUTAGEN ASSAY SYSTEM [J].

APPLEGATE, M ;

JUHN, G ;

MOORE, M ;

HOZIER, J .

MUTATION RESEARCH, 1990, 245 (01) :55-59

[4] MOLECULAR DISSECTION OF MUTATIONS AT THE HETEROZYGOUS THYMIDINE KINASE LOCUS IN MOUSE LYMPHOMA-CELLS [J].

APPLEGATE, ML ;

MOORE, MM ;

BRODER, CB ;

BURRELL, A ;

JUHN, G ;

KASWECK, KL ;

LIN, PF ;

WADHAMS, A ;

HOZIER, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (01) :51-55

[5] EFFECTS OF LOW-DOSE RATE (0.003-0.025 GY/H) CHRONIC X-IRRADIATION ON RADIORESISTANT AND RADIOSENSITIVE L5178Y MOUSE LYMPHOMA-CELLS [J].

BEER, JZ ;

MENCL, J ;

HORNG, MF ;

GREGG, EC ;

EVANS, HH .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1985, 48 (04) :609-619

[6] GENETIC TOXICOLOGY OF THE PHOTOSENSITIZATION OF CHINESE-HAMSTER CELLS BY PHTHALOCYANINES [J].

BENHUR, E ;

FUJIHARA, T ;

SUZUKI, F ;

ELKIND, MM .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1987, 45 (02) :227-230

[7] SINGLE-GENE AND VIABLE CHROMOSOME MUTATIONS AFFECTING THE TK LOCUS IN L5178Y MOUSE LYMPHOMA-CELLS [J].

CLIVE, D ;

HOZIER, J ;

MOORE, MM .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1983, 407 (JUN) :420-422

[8] VALIDATION AND CHARACTERIZATION OF THE L5178Y-TK+/- MOUSE LYMPHOMA MUTAGEN ASSAY SYSTEM [J].

CLIVE, D ;

JOHNSON, KO ;

SPECTOR, JFS ;

BATSON, AG ;

BROWN, MMM .

MUTATION RESEARCH, 1979, 59 (01) :61-108

[9]

CLIVE D, 1990, MUTAGENESIS, V5, P197

[10] SPECIFIC-LOCUS MUTATIONS INDUCED IN EUKARYOTES (ESPECIALLY MAMMALIAN-CELLS) BY RADIATION AND CHEMICALS - A PERSPECTIVE [J].

DEMARINI, DM ;

BROCKMAN, HE ;

DESERRES, FJ ;

EVANS, HH ;

STANKOWSKI, LF ;

HSIE, AW .

MUTATION RESEARCH, 1989, 220 (01) :11-29

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