GENETIC DIVERSITY OF SCHISTOSOMA-MANSONI - QUANTIFYING STRAIN HETEROGENEITY USING A POLYMORPHIC DNA ELEMENT

被引：32

作者：

MINCHELLA, DJ ^{[1
]}

LEWIS, FA ^{[1
]}

SOLLENBERGER, KM ^{[1
]}

WILLIAMS, JA ^{[1
]}

机构：

[1] BIOMED RES INST, ROCKVILLE, MD 20852 USA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1994年 / 68卷 / 02期

关键词：

SCHISTOSOMA MANSONI; GENETIC VARIATION; DNA PROBE PSM750;

D O I：

10.1016/0166-6851(94)90175-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intraspecific genetic variation among 14 geographic isolates of Schistosoma mansoni was quantified using a molecular marker to examine individual genotypes. Genetic crosses demonstrated maternal inheritance of S. mansoni DNA element pSM750. This element revealed diagnostic banding profiles, which allowed accurate strain identification. Most strains had similarity indices greater than 0.75 indicating that within-strain variation in these laboratory parasite populations was low. However, individual parasites from the NMRI strain were quite diverse (S = 0.40). Genetic heterogeneity among strains was quantified using a phenogram of mean genetic distance. Strain diversity between two geographic regions was quantified using a similarity index and was estimated to be substantial among isolates collected from a single local site.

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页码：307 / 313

页数：7

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