ELIMINATION OF POTASSIUM CHANNEL EXPRESSION BY ANTISENSE OLIGONUCLEOTIDES IN A PITUITARY CELL-LINE

被引：26

作者：

CHUNG, SW ^{[1
]}

SAAL, DB ^{[1
]}

KACZMAREK, LK ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,DEPT PHARMACOL,NEW HAVEN,CT 06520

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 13期

关键词：

D O I：

10.1073/pnas.92.13.5955

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The clonal rat pituitary cell line GH(4)C(1) expresses the genes for several voltage-dependent potassium channels including Kv1.5 and Kv1.4. Dexamethasone, a glucocorticoid agonist, induces a slowly inactivating potassium current in these cells but does not alter the amplitude of a rapidly inactivating component of potassium current. We have found that the induction of the slowly inactivating current can be blocked by an antisense phosphorothioate deoxyoligonucleotide to the Kv1.5 mRNA sequence. In contrast, antisense deoxyoligonucleotides against Kv1.4 mRNA specifically decrease the expression of the dexamethasone-insensitive rapidly inactivating current. These results demonstrate the usefulness of antisense oligonucleotides in correlating potassium currents with specific potassium channel proteins in the cell types in which they are naturally expressed.

引用

页码：5955 / 5959

页数：5

共 24 条

[1] INHIBITION OF NEURITE POLARITY BY TAU ANTISENSE OLIGONUCLEOTIDES IN PRIMARY CEREBELLAR NEURONS [J].