PARTIAL CHARACTERIZATION OF RAM SPERMATIDAL BASIC NUCLEAR PROTEINS

Several techniques were used to demonstrate that eight spermatidal proteins are present in the nucleus of ram non-round spermatids. Excepting one, they all contain cysteine and are partially intermolecularly cross-linked in the chromatin of non-round spermatids. Ion-exchange chromatography on carboxymethylcellulose shows that most of them have over-all basic charges similar to those of histones. The molecular weights determined by gel filtration in 6M guanidine hydrochloride, range between that of the smallest histone and that of the basic nuclear sperm protein. © 1978.