IS HISTAMINE PRODUCTION BY THE BLASTOCYST REQUIRED FOR IMPLANTATION IN THE RABBIT

被引:38
作者
DEY, SK [1 ]
JOHNSON, DC [1 ]
SANTOS, JG [1 ]
机构
[1] UNIV KANSAS,MED CTR,RALPH L SMITH RES CTR,DEPT PHYSIOL,KANSAS CITY,KS 66103
关键词
D O I
10.1095/biolreprod21.5.1169
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The histamine producing capacity of rabbit blastocysts and uterine endometrial tissues was determined by measuring their histidine decarboxylase activities (HDC). The production rate of 14CO2 using carboxyl labeled L-histidine as substrate was used as the index of HDC. No enzyme was detectable in the 20.000 x g supernatant of endometrial extracts, but significant activity was found in the extracts from Day 5,6 or 7 blastocysts; the greatest activity was found in Day 6 embryos. Extracts of late Day 6 or Day 7 (Day 6 blastocysts cultured for 24 h) embryos appeared to contain an 'inhibitor' of the HDC when measured against the enzyme activity of fetal tissues. The intraluminal injection of DL-α-methylhistidine dihydrochloride (DL-α-MH), a specific inhibitor of HDC, on Day 5 of pregnancy, interrupted implantation; normal implantation rates were found in contralateral horns instilled with the same amount of L-histidine. The inhibiting action of DL-α-MH could be partially overcome by the co-injection of L-histidine. The results are consistent with the view that rabbit blastocysts have an active histamine forming systen and that inhibition of the enzyme can alter implantation.
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页码:1169 / 1173
页数:5
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