GROWTH AND G1 ARREST OF SARCOMA-VIRUS TRANSFORMED-CELLS IN SERUM FREE MEDIA

被引:8
作者
MOSKOWITZ, M
CHENG, DKS
机构
[1] Department of Biological Sciences, Purdue University, West Lafayette, Indiana
关键词
D O I
10.1002/jcp.1041000320
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Rous‐sarcoma transformed BHK cells can be continuously cultured in a medium containing Eagle's Minimal Essential Medium, iron and biotin. The rate of cell multiplication increased when serine, or serine plus other non‐essential amino‐acids were added to the medium. With biotin deleted from the medium there is a reduction in DNA synthesis and most cells are blocked in G1. Copyright © 1979 Wiley‐Liss, Inc.
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页码:589 / 601
页数:13
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