METABOLIC CORRECTION OF FUCOSIDOSIS LYMPHOID-CELLS BY GALAPTIN-ALPHA-L-FUCOSIDASE CONJUGATES

被引:3
作者
ALLEN, HJ [1 ]
AHMED, H [1 ]
DICIOCCIO, RA [1 ]
机构
[1] ROSWELL PK CANC INST,DEPT GYNECOL ONCOL,BUFFALO,NY 14263
关键词
D O I
10.1016/S0006-291X(05)80214-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To determine if isolated galaptin, an endogenous galactoside-binding lectin, could serve as a transport vehicle of therapeutic agents to cells, galaptin and α-L-fucosidase were coupled using glutaraldehyde. The conjugates were incubated with α-L-fucosidase-deficient, EBV-immortalized lymphoid cells from a fucosidosis patient. Conjugates were effectively bound and internalized by the cells in a lactose inhibitable manner. Internalization of conjugate resulted in the reduced accumulation of α-L-fucosyl-N-acetylglucosaminylasparagine, a glycopeptide that accumulates in cells of fucosidosis patients, to levels found in lymphoid cells from a healthy individual. Thus, galaptin-α-L-fucosidase conjugates may be useful for enzyme replacement therapy of fucosidosis. The concept of using galaptin as a transport vehicle may be applied to the delivery of other compounds to cells bearing galaptin receptors. © 1990 Academic Press, Inc.
引用
收藏
页码:335 / 340
页数:6
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