EVIDENCE FOR A NOVEL ROUTE OF WHEAT STORAGE PROTEINS TO VACUOLES

被引:213
作者
LEVANONY, H
RUBIN, R
ALTSCHULER, Y
GALILI, G
机构
[1] Department of Plant Genetics, Weizmann Institute of Science
关键词
D O I
10.1083/jcb.119.5.1117
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Wheat seed storage proteins are deposited in protein bodies (PB) inside vacuoles, but their subcellular site of aggregation and their route to vacuoles are still controversial. In the present work, an ultra structural analysis of developing wheat endosperm at early to mid maturation was performed to address these issues. Golgi complexes were rarely detected, indicating that their role in wheat storage protein transport is limited. In contrast, a considerable amount of PB was detected in the cytoplasm. Many of these PB were surrounded by RER membranes and were enlarged by fusion of smaller PB. Small, electron lucent vesicles were detected around the surfaces of the PB in the cytoplasm, or attached to them, suggesting that such attachments and subsequent fusion of the vesicles with each other lead to the formation of small vacuoles containing PB inclusions. Immunogold labeling with serum raised against yeast-BiP, an ER-localized protein, demonstrated that the wheat BiP homolog was present within the PB in the cytoplasm as well as inside vacuoles. This confirmed that the PB were formed within the RER and that the Golgi complex was not involved in their transport to vacuoles. It is concluded that a considerable part of the wheat storage proteins aggregate into PB within the RER and are then transported as intact PB to the vacuoles by a novel route that does not utilize the Golgi complex.
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页码:1117 / 1128
页数:12
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共 36 条
  • [1] BECHTEL D B, 1989, Cereal Foods World, V34, P784
  • [2] EARLY STAGES IN WHEAT ENDOSPERM FORMATION AND PROTEIN BODY INITIATION
    BECHTEL, DB
    GAINES, RL
    POMERANZ, Y
    [J]. ANNALS OF BOTANY, 1982, 50 (04) : 507 - 518
  • [3] BECHTEL DB, 1986, CEREAL CHEM, V63, P232
  • [4] POSTTRANSLATIONAL ASSOCIATION OF IMMUNOGLOBULIN HEAVY-CHAIN BINDING-PROTEIN WITH NASCENT HEAVY-CHAINS IN NONSECRETING AND SECRETING HYBRIDOMAS
    BOLE, DG
    HENDERSHOT, LM
    KEARNEY, JF
    [J]. JOURNAL OF CELL BIOLOGY, 1986, 102 (05) : 1558 - 1566
  • [5] DYNAMICS OF VACUOLAR COMPARTMENTATION
    BOLLER, T
    WIEMKEN, A
    [J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1986, 37 : 137 - 164
  • [6] ENDOSPERM MORPHOLOGY AND PROTEIN BODY FORMATION IN DEVELOPING WHEAT-GRAIN
    CAMPBELL, WP
    LEE, JW
    OBRIEN, TP
    SMART, MG
    [J]. AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1981, 8 (01): : 5 - 19
  • [7] REGULATED IMPORT AND DEGRADATION OF A CYTOSOLIC PROTEIN IN THE YEAST VACUOLE
    CHIANG, HL
    SCHEKMAN, R
    [J]. NATURE, 1991, 350 (6316) : 313 - 318
  • [8] STRUCTURAL ASPECTS OF PROTEIN ACCUMULATION IN DEVELOPING PEA COTYLEDONS .1. QUALITATIVE AND QUANTITATIVE CHANGES IN PARENCHYMA CELL VACUOLES
    CRAIG, S
    GOODCHILD, DJ
    HARDHAM, AR
    [J]. AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1979, 6 (01): : 81 - 98
  • [9] THE TOBACCO LUMINAL BINDING-PROTEIN IS ENCODED BY A MULTIGENE FAMILY
    DENECKE, J
    GOLDMAN, MHS
    DEMOLDER, J
    SEURINCK, J
    BOTTERMAN, J
    [J]. PLANT CELL, 1991, 3 (09) : 1025 - 1035
  • [10] CHARACTERIZATION OF HSP-70 COGNATE PROTEINS FROM WHEAT
    GIORINI, S
    GALILI, G
    [J]. THEORETICAL AND APPLIED GENETICS, 1991, 82 (05) : 615 - 620