DIRECT-DETECTION OF LISTERIA-MONOCYTOGENES IN 25 MILLILITERS OF RAW-MILK BY A 2-STEP PCR WITH NESTED PRIMERS

被引：71

作者：

HERMAN, LMF

DEBLOCK, JHGE

MOERMANS, RJB

机构：

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 02期

关键词：

D O I：

10.1128/AEM.61.2.817-819.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes.

引用

页码：817 / 819

页数：3

共 18 条

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