CONCENTRATION OF LIVE RETROVIRUS WITH A REGENERATED CELLULOSE HOLLOW-FIBER, BMM

被引:18
作者
MAKINO, M
ISHIKAWA, G
YAMAGUCHI, K
OKADA, Y
WATANABE, K
SASAKIIWAKI, Y
MANABE, S
HONDA, M
KOMURO, K
机构
[1] ASAHI CHEM IND CO,DEPT BMM DEV & BUSINESS PROMOT,TOKYO,JAPAN
[2] YAMAGUCHI UNIV,SCH MED,INST LAB ANIM,TOKYO,JAPAN
[3] NATL INST HLTH,AIDS RES CTR,TOKYO 141,JAPAN
关键词
D O I
10.1007/BF01309456
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A concentrated live retrovirus is required for in vitro experiments. A cuprammonium-regenerated cellulose hollow fiber, termed BMM, originally developed for biohazardous viral removal, was used to concentrate two different retroviruses, an ecotropic murine leukemia virus (MuLV) and human immunodeficiency virus (HIV). The BMM was useful for concentrating live virus suspension 10- to 30-fold from 500-1000 mi of culture supernatant. The ecotropic MuLV concentrated by BMM was demonstrated to be viable and biologically intact by XC plaque-forming assay and reverse transcriptase assay. The concentrated MuLV reached a much higher titer in the spleen in mice than the original one. The virus concentration assessed by p24 antigen for HIV was clearly higher than that of the original culture supernatant of HIV-infected cell lines. Since BMM hollow fibers trapped viruses by the sieving mechanism but not by adsorption, the viral particles were recovered by washing and the total live virus recovery rate was high, about 50%. Furthermore 60 min sufficed to handle 1000 mi of supernatant in the case of a filtration area of 0.03 m(2). These results show that the BMM provides us with a rapid, safe and efficient method for concentrating live retroviruses.
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页码:87 / 96
页数:10
相关论文
共 18 条
[1]   DEFECTIVE VIRUS IS ASSOCIATED WITH INDUCTION OF MURINE RETROVIRUS-INDUCED IMMUNODEFICIENCY SYNDROME [J].
CHATTOPADHYAY, SK ;
MORSE, HC ;
MAKINO, M ;
RUSCETTI, SK ;
HARTLEY, JW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (10) :3862-3866
[2]   CHARACTERISTICS AND CONTRIBUTIONS OF DEFECTIVE, ECOTROPIC, AND MINK CELL FOCUS-INDUCING VIRUSES INVOLVED IN A RETROVIRUS-INDUCED IMMUNODEFICIENCY SYNDROME OF MICE [J].
CHATTOPADHYAY, SK ;
SENGUPTA, DN ;
FREDRICKSON, TN ;
MORSE, HC ;
HARTLEY, JW .
JOURNAL OF VIROLOGY, 1991, 65 (08) :4232-4241
[3]   GENETIC-STUDY OF LYMPHOMA INDUCTION BY AKR MINK CELL FOCUS-INDUCING VIRUS IN AKR X NFS CROSSES [J].
CLOYD, MW ;
HARTLEY, JW ;
ROWE, WP .
JOURNAL OF EXPERIMENTAL MEDICINE, 1981, 154 (02) :450-458
[4]   A NOVEL METHOD FOR REMOVAL OF HUMAN IMMUNODEFICIENCY VIRUS - FILTRATION WITH POROUS POLYMERIC MEMBRANES [J].
HAMAMOTO, Y ;
HARADA, S ;
KOBAYASHI, S ;
YAMAGUCHI, K ;
IIJIMA, H ;
MANABE, S ;
TSURUMI, T ;
AIZAWA, H ;
YAMAMOTO, N .
VOX SANGUINIS, 1989, 56 (04) :230-236
[5]   CLONAL CELL LINES FROM A FERAL MOUSE EMBRYO WHICH LACK HOST-RANGE RESTRICTIONS FOR MURINE LEUKEMIA VIRUSES [J].
HARTLEY, JW ;
ROWE, WP .
VIROLOGY, 1975, 65 (01) :128-134
[6]  
JOHNSON VA, 1990, TECHNIQUES HIV RES, P98
[7]   MURINE ACQUIRED-IMMUNODEFICIENCY-SYNDROME (MAIDS) - AN ANIMAL-MODEL TO STUDY THE AIDS PATHOGENESIS [J].
JOLICOEUR, P .
FASEB JOURNAL, 1991, 5 (10) :2398-2405
[8]  
MAKINO M, 1990, J IMMUNOL, V144, P4347
[9]   SOME MORPHOLOGICAL-CHARACTERISTICS OF POROUS POLYMERIC MEMBRANES PREPARED BY MICROPHASE SEPARATION METHOD [J].
MANABE, S ;
KAMATA, Y ;
IIJIMA, H ;
KAMIDE, K .
POLYMER JOURNAL, 1987, 19 (04) :391-404
[10]  
MANABE S-C, 1989, Membrane, V14, P77