ANALYSIS OF THE CYTOSOLIC HSP70 GENE FAMILY IN ZEA-MAYS

被引：16

作者：

BATES, EEM

VERGNE, P

DUMAS, C

机构：

[1] Ecole Normale Superieure de Lyon, Reconnaissance Cellulaire et Amelioration des Plantes, UMR 9938, CNRS-INRA, Lyon cedex 07, 69364

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 25卷 / 05期

关键词：

HEAT SHOCK 70 KDA PROTEIN; MULTIGENE FAMILY; POLYMERASE CHAIN REACTION; ZEA MAYS;

D O I：

10.1007/BF00028885

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study we have analysed the multigene family coding for the cytoplasmic heat shock 70 kDa proteins (hsp70) in Zea mays. Fully degenerate primers were used in a polymerase chain reaction (PCR) to amplify selected regions of the hsp70 genes. Sequence and Southern blot analysis reveals that at least three highly conserved genes exist in maize. In addition, amplification reveals the presence of a conserved intron in all genes examined. Expression analysis shows that the hsp70 genes studied represent members of the inducible and constitutive families. The results obtained may indicate that there are subfamilies of cytoplasmic hsp70 genes expressed in higher plants.

引用

页码：909 / 916

页数：8

共 25 条

[1] EXPRESSION OF THE GENES ENCODING HSP73, HSP18, AND UBIQUITIN IN RADICLES OF HEAT-SHOCKED MAIZE SEEDLINGS
ATKINSON, BG
LIU, L
GOPING, IS
WALDEN, DB
[J]. GENOME, 1989, 31 (02) : 698 - 704
[2] BARLOY D, 1989, MAYDICA, V34, P303
[3] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[4] ESSENTIAL ROLES OF 70KDA HEAT INDUCIBLE PROTEINS
CRAIG, EA
[J]. BIOESSAYS, 1989, 11 (2-3) : 48 - 52
[5] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY
FEINBERG, AP
VOGELSTEIN, B
[J]. ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) : 6 - 13
[6] PROTEIN FOLDING IN THE CELL
GETHING, MJ
SAMBROOK, J
[J]. NATURE, 1992, 355 (6355) : 33 - 45
[7] HEAT-SHOCK PROTEINS AND THEIR MESSENGER-RNAS IN DRY AND EARLY IMBIBING EMBRYOS OF WHEAT
HELM, KW
ABERNETHY, RH
[J]. PLANT PHYSIOLOGY, 1990, 93 (04) : 1626 - 1633
[8] THE HEAT-SHOCK RESPONSE OF POLLEN AND OTHER TISSUES OF MAIZE
HOPF, N
PLESOFSKYVIG, N
BRAMBL, R
[J]. PLANT MOLECULAR BIOLOGY, 1992, 19 (04) : 623 - 630
[9] JORGENSEN JA, 1992, PLANT CELL REP, V11, P576, DOI 10.1007/BF00233096
[10] SEQUENCES OF 2 HSC 70 CDNAS FROM LYCOPERSICON-ESCULENTUM
LIN, TY
DUCK, NB
WINTER, J
FOLK, WR
[J]. PLANT MOLECULAR BIOLOGY, 1991, 16 (03) : 475 - 478

← 1 2 3 →