MUTATIONAL ANALYSIS OF PC1 (SPC3) IN PC12 CELLS - 66-KDA PC1 IS FULLY FUNCTIONAL

被引:37
作者
ZHOU, Y
ROVERE, C
KITABGI, P
LINDBERG, I
机构
[1] LOUISIANA STATE UNIV, MED CTR, DEPT BIOCHEM & MOLEC BIOL, NEW ORLEANS, LA 70112 USA
[2] CNRS, INST PHARMACOL, F-06560 VALBONNE, FRANCE
关键词
D O I
10.1074/jbc.270.42.24702
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The proteinase mPC1, a neuroendocrine member of the mammalian family of subtilisin-like enzymes, has previously been shown to be converted to a carboxyl-terminally truncated 66-kDa form during transport through the secretory pathway. The cleavage site and the function of this carboxyl-terminal truncation event are unknown. We have performed site-directed mutagenesis of two paired basic sites in the mPC1 carboxyl-terminal tail and expressed these constructs in PC12 cells, a rat pheochromocytoma known to lack endogenous PC1. We found that the most likely site for the truncation event was at Arg(590)-Arg(591) since mutation of this site to Lys-His prevented processing of 87-kDa PC1. APC1 mutant carboxyl-terminally truncated at this site and expressed in PC12 cells was efficiently routed to the secretory pathway and stored in secretory granules, indicating that the carboxyl-terminal extension is not required for sorting of this enzyme. The function of the various PC1 constructs was assessed by analyzing proneurotensin cleavage to various forms. The carboxyl-terminally truncated PC1 mutant was found to perform most of the cleavages of this precursor as well as wild-type PC1; however, the blockade mutant processed proneurotensin much less efficiently, Differences between the site preferences of the various enzymes were noted. Our results support the notion that carboxyl terminal processing of PC1 serves to regulate PC1 activity.
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页码:24702 / 24706
页数:5
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