IDENTIFICATION OF LEGUME NODULE BACTEROIDS BY FLUORESCENT ANTIBODY REACTION

被引：36

作者：

TRINICK, MJ

机构：

[1] Department of Soil Science and Plant Nutrition, Institute of Agriculture, University of Western Australia, Nedlands, Western Australia

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1969年 / 32卷 / 02期

关键词：

D O I：

10.1111/j.1365-2672.1969.tb00965.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A fluorescent antibody technique which permits rapid identification of legume nodule bacteroids and which permits the separation of bacteroid material and laboratory grown cultures of close serological affinity is described. Specificity of fluorescein isothiocyanate γ‐globulin conjugates was increased with dilution. The ability of a medium sized clover nodule to produce sufficient bacteroid material for 20 fluorescent antibody stains allows increased accuracy and scope of the method. A single operator in 1 day may test > 400 nodules for one or more symbionts. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：181 / &

共 12 条

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