GEL ISOELECTRIC-FOCUSING OF WHEAT ALCOHOL-DEHYDROGENASE

被引:7
作者
FEJER, O
OROSZFEJER, K
BELEA, A
机构
[1] Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged
关键词
Alcohol dehydrogenase; Hexaploid wheat; Isoelectric focusing; pI values;
D O I
10.1007/BF00265706
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Alcohol dehydrogenase (ADH) of different hexaploid wheat subspecies and varieties was investigated by isoelectric focusing in polyacrylamide gels. With this technique six ADH isoenzymes can be separated, while by the standard electrophoretic technique only three are visible. The ADH pattern revealed by isoelectric focusing is in full accordance with the hypothesis that the active ADH isozymes in hexaploid wheat are dimers composed of six possible combinations of subunits coded by triplicate structural genes. © 1979 Springer-Verlag.
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页码:37 / 39
页数:3
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