INVESTIGATION OF THE MICROENVIRONMENT POLARITY OF A CHROMATOGRAPHIC SURFACE USING TOTAL INTERNAL-REFLECTION FLUORESCENCE

被引：15

作者：

RANGNEKAR, VM ^{[1
]}

OLDHAM, PB ^{[1
]}

机构：

[1] MISSISSIPPI STATE UNIV, DEPT CHEM, MISSISSIPPI STATE, MS 39762 USA

来源：

ANALYTICAL CHEMISTRY | 1990年 / 62卷 / 11期

关键词：

D O I：

10.1021/ac00210a010

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A planar chromatographic surface was studied for microenvironmental polarity by using total internal reflection fluorescence (TIRF). Pyrene was adsorbed on two different chemically modified quartz plates. The surfaces of the modified quartz plates were covered with either a monomeric C18 or a polymeric C18 layer. TIRF data were obtained for the adsorbed pyrene in the presence of different polar solvents. As the polarity of the solvents increased, an overall increase in fluorescence intensity was observed and a change in the relative vibronic band intensities (III/I) of pyrene was noted. Also, variable-angle TIRF data were collected for the adsorbed pyrene In the presence of a 40% methanol/water mixture (v/v). The fluorescence contribution from the bulk fluorescence was found to decrease as the incidence angle increased, as was expected. © 1990, American Chemical Society. All rights reserved.

引用

页码：1144 / 1147

页数：4

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