COMPARATIVE-STUDIES OF PROTEIN-BIOSYNTHESIS - THE MAIN EXPERIMENTAL PARAMETERS IN PULSE AND PULSE-CHASE EXPERIMENTS MUST BE STANDARDIZED

被引：2

作者：

APPEL, M ^{[1
]}

COUDERC, E ^{[1
]}

FEGER, J ^{[1
]}

机构：

[1] UNIV PARIS SUD,UFR SCI PHARMACEUT,BIOCHIM LAB,5 RUE JB CLEMENT,F-92296 CHATENAY MALABRY,FRANCE

来源：

BIOLOGY OF THE CELL | 1992年 / 74卷 / 02期

关键词：

HEPATOCYTE; PULSE; CHASE; PROTEIN BIOSYNTHESIS;

D O I：

10.1016/0248-4900(92)90031-U

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The influence of experimental conditions was investigated in pulse and pulse-chase experiments involving L-[S-35]methionine incorporation by isolated rat hepatocytes. The incorporation of the labelled amino acid must be linear as a function of dose and time, and similar hepatocyte densities should be used to compare radioactive uptake. High hepatocyte densities give greater precision. In pulse-chase experiments, it was shown that a pool of free radioactivity moves progressively during the chase phase from the intracellular compartment. Protein-associated radioactivity must therefore be expressed as a function of both total radioactive uptake and total labelled protein.

引用

页码：235 / 238

页数：4

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