QUANTITATIVE-DETERMINATION OF CARDIAC MYOSIN SUBUNITS STAINED WITH FAST GREEN IN SDS-ELECTROPHORESIS GELS

被引：6

作者：

MEDUGORAC, I

机构：

[1] Physiologisches Institut,-Lehrstuhl II-der Universität Tübingen, Tübingen, 7400

来源：

BASIC RESEARCH IN CARDIOLOGY | 1979年 / 74卷 / 04期

关键词：

D O I：

10.1007/BF01908393

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

In the present study a procedure is described which permits simultaneous relative quantification of all myosin subunits in the same fast green stained electrophoresis gel. The relative protein quantification can be performed successively in the same gel using densitometry and the elution method, respectively. Thus, the results obtained with one method can be checked with the other. This procedure provided reliable estimates of the stoichiometry of cardiac myosin subunits within a range of about 20-110 μg of total myosin protein per electrophoresis gel. © 1979 Dr. Dietrich Steinkopff Verlag.

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页码：406 / 416

页数：11

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