LARGE-SCALE PREPARATION OF AN IMMUNOCONJUGATE CONSTRUCTED WITH HUMAN RECOMBINANT CD4 AND DEGLYCOSYLATED RICIN-A CHAIN

被引：11

作者：

GHETIE, V ^{[1
]}

TILL, MA ^{[1
]}

GHETIE, MA ^{[1
]}

UHR, JW ^{[1
]}

VITETTA, ES ^{[1
]}

机构：

[1] UNIV TEXAS,SW MED CTR,DEPT MICROBIOL,5323 HARRY HINES BLVD,DALLAS,TX 75235

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 126卷 / 01期

关键词：

CD4; Human immunodeficiency virus; Immunoconjugate; Ricin A chain;

D O I：

10.1016/0022-1759(90)90021-M

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the preparation and purification of large amounts (grams) of a conjugate containing recombinant CD4 antigen (rCD4) and chemically deglycosylated ricin A chain (dgA) is described. The cross-linking of rCD4 and dgA molecules was accomplished with N-succinimidyl-oxycarbonyl-α-methyl-(2-pyridyldithio)toluene (SMPT). The rCD4-dgA conjugate was purified by an automatic liquid chromatography system consisting of Blue Sepharose CL-4B and Sephacryl S-200HR Pharmacia Bioprocess columns. The purified, endotoxin-free rCD4-dgA conjugate had a stable (hindered) disulfide bond between rCD4 and dgA and was able to efficiently kill a human T cell line infected HIV-1. © 1990.

引用

页码：135 / 141

页数：7

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