Low levels of some heavy metals and inorganic substances have been detected in the Great Lakes ecosystem; these include arsenic, cadmium, chromium, copper, iron, mercury, nickel, selenium, zinc and fluoride. The levels of individual elements may be below those which could cause toxic effects, but the potential toxicity of simultaneous exposure to a combination of these elements remains unknown. This paper reports on a study designed to investigate the toxic effects of a combination of inorganic substances in the rat when administered at levels equal or greater than the objectives established in the Great Lakes Water Quality Agreement (1978).