IDENTIFICATION OF A FIBROBLAST-DERIVED EPITHELIAL MORPHOGEN AS HEPATOCYTE GROWTH-FACTOR

被引：1158

作者：

MONTESANO, R ^{[1
]}

MATSUMOTO, K ^{[1
]}

NAKAMURA, T ^{[1
]}

ORCI, L ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC SCI,DEPT BIOL,FUKUOKA 812,JAPAN

来源：

CELL | 1991年 / 67卷 / 05期

关键词：

D O I：

10.1016/0092-8674(91)90363-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously shown that Madin-Darby canine kidney (MDCK) epithelial cells grown in collagen gels in the presence of fibroblasts or fibroblast-conditioned medium (CM) form branching tubules, instead of the spherical cysts that develop under control conditions. We now report that the fibroblast-derived molecule responsible for epithelial tubulogenesis is hepatocyte growth factor (HGF). First, addition of exogenous HGF to cultures of MDCK cells induces formation of epithelial tubules. Second, the tubulogenic activity of fibroblast CM is completely abrogated by antibodies to HGF. These results demonstrate that HGF, a polypeptide that was identified as a mitogen for cultured hepatocytes, has the properties of a paracrine mediator of epithelial morphogenesis, and suggest that it may play important roles in the formation of parenchymal organs during embryonic development.

引用

页码：901 / 908

页数：8

共 57 条

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BERNFIELD M., 1984, ROLE EXTRACELLULAR M, P545

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