BINDING SELECTIVITY OF RHIZOXIN, PHOMOPSIN-A, VINBLASTINE, AND ANSAMITOCIN P-3 TO FUNGAL TUBULINS - DIFFERENTIAL INTERACTIONS OF THESE ANTIMITOTIC AGENTS WITH BRAIN AND FUNGAL TUBULINS

被引：11

作者：

LI, Y ^{[1
]}

KOBAYASHI, H ^{[1
]}

HASHIMOTO, Y ^{[1
]}

IWASAKI, S ^{[1
]}

机构：

[1] UNIV TOKYO,INST APPL MICROBIOL,1-1-1 YAYOI,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 187卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)91255-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The binding of four potent antimitotic agents, rhizoxin (RZX), phomopsin A (PMS-A), ansamitocin P-3 (ASMP-3), and vinblastine (VLB), to tubulins from RZX-sensitive and -resistant strains of Aspergillus nidulans, Schizosaccharomyces pombe, and Saccharomyces cerevisiae was investigated. Mycelial extracts to which RZX could bind contained β-tubulin with Asn as the 100th amino acid residue (Asn-100) in all cases, and those without affinity for RZX contained β-tubulins with either Ile-100 or Val-100. Though PMS-A shares the same binding site as RZX and ASMP-3 on porcine brain tubulin (Asn-100), only ASMP-3 bound Asn-100 fungal tubulins in a competitive manner with respect to RZX. PMS-A and VLB, which strongly bind to porcine brain tubulin, did not bind to any of the fungal mycelial extracts examined. The results indicate differential interactions of these antimitotic agents with brain and fungal tubulins. © 1992.

引用

页码：722 / 729

页数：8

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